Sexual and somatic hybridization

Retrotransposon-based markers from potato monoploids used in somatic hybridization.

In an attempt to remove lethal and deleterious genes and enhance the heterozygosity of the potato genome, we developed several diverse somatic hybrids through the electrofusion of selected monoploids. Somatic hybrids and somaclones resulting from fused and unfused protoplasts, respectively, were verified with microsatellites. Molecular markers anchored in the Tst1 retrotransposon were used to e...

Genomic changes at the early stage of somatic hybridization.

A broad spectrum of genetic and epigenetic changes is induced by wide hybridization and subsequent polyploidization, but the timing of these events remains obscure because early hybrid cells are very difficult to harvest and analyze. Here, we used both cytological and genetic marker approaches to analyze the constitution of very young somatic hybrid cells between japonica rice (Oryza sativa L. ...

DM Domain Genes: Sexual and somatic development during vertebrate embryogenesis

Interrelationships among measures of somatic, skeletal, dental, and sexual maturity.

The interrelationships among five measures of physiologic maturity for 50 French-Canadian girls are evaluated--menarche, peak height velocity (PHV), 75% skeletal maturity, appearance of the ulnar sesamoid, and 90% dental development. The mean ages of occurrence of the events differed significantly (P less than 0.01). Menarche and 90% dental maturity showed the least variability. Ages of PHV, me...

Observations and Suggestions for Improving Somatic Hybridization by Plant Protoplast Isolation, Fusion, and Culture

De (Triticum aestivum): In vitro production and utilization of doubled haploids, p. 101–124. In: Y.P.S. Bajaj (ed.). Biotechnology in agriculture and forestry. vol. 12: Haploids in crop improvement I. Springer-Verlag, Berlin. Powell, W., P.D.S. Caligari, and J.M. Dunwell. 1986. Field performance of lines derived from haploid and diploid tissues of Hordeum vulgare. Theor. Applied Genet. 72:458–4...